Step 3: Egg retrieval and culture
On the day of the retrieval we will need a semen specimen which needs to be collected at the clinic. A collection room is available for privacy. Those who get nervous can provide sample beforehand at their convenient time. Sample is kept freeze for future use on the day of procedure. Female partner will come in empty stomach as G/A may needed. Patient needs to come at least 30 minutes before retrieval. The retrieval procedure takes approximately 10-15 minutes and patient can go to recovery room within 30 minutes. The procedure involves a transvaginal ultrasound with a needle guide that is used to aspirate the fluid from within the follicles under real time visualization. The oocytes are identified in the follicular fluid by an embryologist. The number of eggs will be known shortly after the completion of the procedure.
The recovery usually takes less than 30 minutes. Patient will be given discharge instructions by medical officer. Patients are allowed to call medical officer further clarification if needed. The next day fertilization is checked and result is informed to the patient. 100% of the oocytes retrieved are not usually fertilized. There is no good way to predict how many eggs will actually fertilize.
Step 4: Embryo transfer
Depending on several factors, including number of fertilized eggs and age, the embryos will be transferred into the uterus on either day 2, 3 or at the blastocyst stage (5-6). Egg retrieval day is considered day 0.
Eight cells grade 1 embryo